Skip to main content
. Author manuscript; available in PMC: 2009 Nov 1.
Published in final edited form as: Arch Biochem Biophys. 2008 Aug 20;479(1):63–68. doi: 10.1016/j.abb.2008.08.009

Fig. 4. The YQTI peptide, but not the mutant AQTA peptide induces oligomerization of AP-1 in solution.

Fig. 4

(A) Bovine adrenal cytosol was incubated with either the YQTI or the mutant ATQA peptides (250 µM) for 1 hour on ice and loaded directly onto 10–25 % linear sucrose gradients for centrifugation. Fractions were collected and analyzed by SDS-PAGE and western blotting as described in the Experimental Procedures. (B) Similar experiments were performed using cytosol incubated with various concentrations of the YQTI peptides. The additional band on the top left shows 3% input.