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. Author manuscript; available in PMC: 2009 Nov 1.
Published in final edited form as: Arch Biochem Biophys. 2008 Aug 20;479(1):63–68. doi: 10.1016/j.abb.2008.08.009

Fig. 5. Polymerization of purified AP-1 in solution does not require cytosolic proteins.

Fig. 5

Purified AP-1 (15 nM) was incubated with either the YQTI or the mutant AQTA peptides at the indicated concentrations for 1 hour on ice followed by centrifugation for 15 min at 40,000 rpm in a TLA.100.3 rotor. The resulting pellets were analyzed by SDS-PAGE and western blotting with 100/3 antibody (lane 1 contains 20 % input).