Figure 4.

A combined, multi-scale NEURON and GENESIS model. (A) Setup of combined model, using NEURON and GENESIS model definition files. (B) Information flow during simulation. The two models were run independently for an initial settling period and for the test pulse to the NEURON model. During the combined simulation phase, each model was advanced for 1 s and then data was transferred via Python to the other model. Finally a second test pulse was delivered. (C) Response of NEURON model to first test pulse. (D) Calcium and MAPK levels in the signaling model. (E) Voltage responses from the NEURON model. (F) Experiment design and input to NEURON model. A test current pulse of 0.15 nA was delivered for 0.25 s to the NEURON model in the initialization phase. At the start of the combined simulation, a stimulus of 10 nA was delivered for 7 s. After 180 s of combined simulation a second test pulse (0.15 nA, 0.25 s) was applied. (G) Response of NEURON model to second test pulse. The difference is due to modulation of KCa by the elevated MAPK activity.