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. Author manuscript; available in PMC: 2009 Jan 6.
Published in final edited form as: Cancer Ther. 2008;6(B):955–964.

Figure 1.

Figure 1

MUC-1 vaccination transiently increases the percentages of functional CD4 and CD8 T cells in both survivors and non-survivors. Pre and post vaccination PBMCs were thawed, counted, and 106 cells were aliquotted into 96 well plates. The cells were stimulated with PMA and ionomycin for 6 hours at 37°C in the presence of Golgistop and then stained for CD3, CD4, CD8 and intracellular IFN-γ and TNF-α. Percentage of T cells producing IFN is marked by closed circles and the percentage producing TNF is shown in the open squares. V1, V2 and V3 correspond to three consecutive vaccine administrations.