Skip to main content
. Author manuscript; available in PMC: 2009 Jan 6.
Published in final edited form as: Cancer Ther. 2008;6(B):955–964.

Figure 2.

Figure 2

MUC-1 vaccination transiently increases the percentages of CD8 T cells expressing the effector molecules perforin and Granzyme B. Pre and post vaccination PBMCs were thawed, counted, and 106 cells were aliquotted into 96 well plates. The cells were surface stained for CD3, CD4, and CD8, permeabilized and stained with anti-perforin and anti-granzyme B antibodies. Percentage of cells positive for Granzyme B is marked by a solid line and the percentage positive for perforin is marked by a dashed line. V1, V2 and V3 correspond to three consecutive vaccine administrations.