Figure 3.
Pancreatic cancer patients have a higher percentage of regulatory T cells compared to age-matched healthy controls. PBMCs were thawed, counted and 106 cells were aliquotted into 96 well plates. The cells were surface stained for CD3, CD4, CD8, permeabilized and then incubated with either an isotype control antibody (A) or with an anti-FoxP3 antibody (B). Representative dot plots are shown in A and B and a summary graph of all patients analyzed (normals n=9 and cancer patients n=7) is shown in C. Statistical analysis by a Wilcoxon ranked sum test resulted in a p-value of 0.012.