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. Author manuscript; available in PMC: 2009 Nov 28.
Published in final edited form as: Cell. 2008 Nov 28;135(5):825–837. doi: 10.1016/j.cell.2008.09.059

Figure 3. Lrp5 regulates serotonin synthesis through its expression in gut.

Figure 3

(A–B) Real-time PCR analysis of Lrp5 (A) and Tph1 (B) expression in the gut and long bones (Bone) of Lrp5gut−/− and Lrp5osb−/− compared to +/+;Villin-Cre and +/+;α1(I)Col-Cre mice, respectively.

(C) Serum serotonin levels in +/+;Villin-Cre, Lrp5gut−/−, +/+;α1(I)Col-Cre and Lrp5osb−/− mice.

(D) Histomorphometric analysis of vertebrae of WT, +/+;Villin-Cre, Lrp5gut−/−, Lrp5KIgut+/−, +/+;α1(I)Col-Cre, Lrp5osb−/− and Lrp5KIosb+/− mice.

(E) Real-time PCR analysis of Cyclins and Col1a1 expression in long bones of WT, Lrp5gut−/− and Lrp5osb−/− mice.

(F) In vivo osteoblast proliferation in WT, Lrp5gut−/− and Lrp5osb−/− mice.

(G) Western blot analysis of Lrp5 high bone mass (G171V) cDNA expression (Flag) in bone and gut of Lrp5KIosb+/− and Lrp5KIgut+/− compared to +/+;α1(I)Col-Cre and +/+;Villin-Cre mice respectively.

(H–I) Real-time PCR analysis of Tph1 expression (H) in the gut and long bones (Bone) and serum serotonin levels (I) in Lrp5KIosb+/− and Lrp5KIgut+/− compared to +/+;Villin-Cre and +/+;α1(I)Col-Cre mice, respectively.

(J–K) Real-time PCR analysis of Cyclins and Col1a1 expression (J) and in vivo osteoblast proliferation (K) in long bones of WT, Lrp5KIgut+/− and Lrp5KIosb+/− mice.

(L) Plasma serotonin levels in two high bone mass (HBM) patients and age matched controls (n=3).