(A–B) Real-time PCR analysis of Lrp5 (A) and Tph1 (B) expression in the gut and long bones (Bone) of Lrp5gut−/− and Lrp5osb−/− compared to +/+;Villin-Cre and +/+;α1(I)Col-Cre mice, respectively.
(C) Serum serotonin levels in +/+;Villin-Cre, Lrp5gut−/−, +/+;α1(I)Col-Cre and Lrp5osb−/− mice.
(D) Histomorphometric analysis of vertebrae of WT, +/+;Villin-Cre, Lrp5gut−/−, Lrp5KIgut+/−, +/+;α1(I)Col-Cre, Lrp5osb−/− and Lrp5KIosb+/− mice.
(E) Real-time PCR analysis of Cyclins and Col1a1 expression in long bones of WT, Lrp5gut−/− and Lrp5osb−/− mice.
(F) In vivo osteoblast proliferation in WT, Lrp5gut−/− and Lrp5osb−/− mice.
(G) Western blot analysis of Lrp5 high bone mass (G171V) cDNA expression (Flag) in bone and gut of Lrp5KIosb+/− and Lrp5KIgut+/− compared to +/+;α1(I)Col-Cre and +/+;Villin-Cre mice respectively.
(H–I) Real-time PCR analysis of Tph1 expression (H) in the gut and long bones (Bone) and serum serotonin levels (I) in Lrp5KIosb+/− and Lrp5KIgut+/− compared to +/+;Villin-Cre and +/+;α1(I)Col-Cre mice, respectively.
(J–K) Real-time PCR analysis of Cyclins and Col1a1 expression (J) and in vivo osteoblast proliferation (K) in long bones of WT, Lrp5KIgut+/− and Lrp5KIosb+/− mice.
(L) Plasma serotonin levels in two high bone mass (HBM) patients and age matched controls (n=3).