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. Author manuscript; available in PMC: 2009 Nov 28.
Published in final edited form as: Cell. 2008 Nov 28;135(5):825–837. doi: 10.1016/j.cell.2008.09.059

Figure 5. Serotonin inhibits osteoblasts proliferation through Htr1b.

Figure 5

(A–B) Real-time PCR analysis of serotonin receptors expression in primary osteoblasts (A) and of Htr1b expression in different tissues of WT mice (B).

(C–E) Histomorphometric analysis of vertebrae of 3 month-old WT, Htr2a−/− (C), Htr2bosb−/− (D) and Htr1b−/− (E) mice.

(F) Real-time PCR analysis of Cyclins, Runx2, Osx, and Atf4 expression in long bones of WT, Htr1b−/−, Htr2a−/− and Htr2bosb−/− mice.

(G) Real-time PCR analysis of CycD1 expression in primary osteoblasts of WT, Htr1b−/−, Htr2a−/− and Htr2bosb−/− mice treated with serotonin (50μM).

(H) In vivo osteoblast proliferation in WT, Htr1b−/−, Htr2a−/− and Htr2bosb−/− mice.

(I) Histomorphometric analysis of vertebrae of 6 week-old WT and Htr1bosb+/− mice.