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. Author manuscript; available in PMC: 2009 Nov 28.
Published in final edited form as: Cell. 2008 Nov 28;135(5):825–837. doi: 10.1016/j.cell.2008.09.059

Figure 6. Lrp5 mediates its effect through CREB.

Figure 6

(A) Histomorphometric analysis of vertebrae of WT, single (Lrp5+/−, Runx2+/−, Osx+/−, Atf4+/− or Crebosb+/−) and double (Lrp5+/−;Runx2+/−, Lrp5+/−;Osx+/−, Lrp5+/−;Atf4+/− or Lrp5+/−;Crebosb+/−) heterozygous mutant mice.

(B–C) Western blot analysis of CREB phosphorylation (B) and chromatin-immunoprecipitation assay of CREB binding to the consensus cAMP response element (CRE) in CycD1 promoter (C) at 0, 3, 6, 12 and 24 hours upon serotonin (50μM) treatment. Coding sequence (CDS) PCR, IgG pulldown and ATF4 binding to the same site were used as negative controls.

(D) Real-time PCR analysis of CycD1 expression in the long bones of WT, Lrp5+/−, Lrp5−/−, Crebosb+/−, Osx+/− and Runx2+/− mice.

(E) Real-time PCR analysis of Creb expression in the long bones of WT, Lrp5−/−, Lrp5gut−/−, Lrp5osb−/−, Lrp5KIgut+/− and Lrp5KIosb+/− mice.