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. Author manuscript; available in PMC: 2009 Dec 12.
Published in final edited form as: Biochem Biophys Res Commun. 2008 Oct 16;377(2):636–641. doi: 10.1016/j.bbrc.2008.10.030

Figure 3. δ-catenin overexpression does not induce any significant changes in RhoA activity in HEK293 cells.

Figure 3

(A) HEK293 cell lysates were subjected to RhoA assay to analyze cellular RhoA activity. (B) HEK293 cells were incubated without serum for overnight, and cells were incubated either with or without serum for additional 30 min, then harvested and subjected to RhoA assay. (C) Levels of GTP-RhoA were measured in HEK293 cells transfected with either mock or GFP-δ-catenin. (D) Relative activity of RhoA was determined as described under “Experimental procedures”. Number under graph is identical to the lane number in Figure 3C. No significant change was observed.