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. Author manuscript; available in PMC: 2009 Nov 1.
Published in final edited form as: Mol Genet Metab. 2008 Sep 20;95(3):133–141. doi: 10.1016/j.ymgme.2008.08.003

FIG. 3. Metabolic cooperativity demonstrated by uptake of secreted AC by non-transduced Farber fibroblasts.

FIG. 3

FIG. 3

(A) Non-transduced (NT) Farber fibroblasts were overlaid with media harvested from the indicated cells and incubated for 48 h. The cells were then pulsed with [3H-ceramide]-sphingomyelin for 24 h and lipids analyzed by TLC. Error bars represent SD; measurements are averages of three independent experiments. * p < 0.05, ** p < 0.01, for groups indicated versus the non-transduced (NT) control. (B) Normal, NT and transduced (RV/AC/huCD25 or LV/AC/huCD25) Farber fibroblasts were grown in serum-free culture medium. After 24 h, both cells and media were collected. Proteins were separated by PAGE and immunoblotted with an anti-acid ceramidase antibody.