Figure 3.

Central memory CD4⁺ T cells generated by Leishmania infection require IL-12 to become IFN-γ producers. CD4⁺ T cells from immune C57BL/6 mice were sorted into naïve (CD62L^high CD44^low), effector (CD62L^low CD44^high), or central memory (CD62L^high CD44^high) and cultured at a 1:5 ratio with splenocytes from naïve Thy disparate animals, and stimulated with FTAg alone or in the presence of IL-12 and αIL-4. After 4 days cells were harvested and stained for CD4 and IFN-γ. Numbers represent percentage of CD4⁺ IFN-γ⁺ cells and plots are representative of two experiments.