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. Author manuscript; available in PMC: 2009 Aug 1.
Published in final edited form as: Nat Cell Biol. 2008 Jul 6;10(8):979–986. doi: 10.1038/ncb1758

Figure 5. Phosphorylation on tyrosine 310 regulates TJ formation by dissociating ephrinB1 from Par-6.

Figure 5

(a) Each blastomere of 2-cell stage embryos was injected with 200 pg of ephrinB1WT-HA or ephrinB1Y310F-HA and 200 pg of FGFR1 KE or FGFR1 KD RNAs where indicated. Embryos were sectioned and immunostained for ZO-1 and ephrinB1 (HA). Arrows indicate the ZO-1 localization in TJs. The scale bar corresponds to 1 µm. (b) Each blastomere of 2-cell stage embryos was injected with 20 ng of ControlMO or ephrinB1MO and 150 pg of ephrinB1WTΔUTR-HA or ephrinB1Y310ΔUTR-HA RNAs where indicated. Embryos were sectioned and immunostained for ZO-1 and ephrinB1 (HA). Arrows indicate the ZO-1 localization in TJs. The scale bar corresponds to 1 µm. (c) Each blastomere of 2-cell stage embryos was injected with 200 pg of ephrinB1-HA or ephrinB1Y310-HA and 200 pg of FGFR1 KE RNAs where indicated. At stage 8.5, ectodermal explants were dissected, and harvested at stage 10.5. Lysates of these explants were analyzed directly by SDS-PAGE and immunoblotted with indicated antibodies.