. Author manuscript; available in PMC: 2009 Sep 1.

Published in final edited form as: J Pathol. 2008 Sep;216(1):120–128. doi: 10.1002/path.2392

Table 1.

Pattern of expression of tight junction proteins in normal mouse renal tubule and in cysts of Pkd1 nl/nl mice

Tight junction¹ protein	Normal wt/wt		Pkd1 nl/nl

	Tubule segments²	Subcellular location³	Cyst type⁴	Subcellular location²	Expression level⁵
ZO-1	All	TJ	S = I = L	TJ	2
Occludin	All	TJ	S = I = L	TJ	2
Claudin-1	BC	?	Very rare L	Bl	2
Claudin-2	PT, uTDL	TJ	-	-	0
Claudin-3	TALH, DCT, CD	Bl	S ≫ I, L	Bl	+/−
Claudin-4	ThAL, CD	TJ	S ≫ I, L	Bl	1
Claudin-7	lTDL, DCT, CNT, CD	TJ, Bl	S = I ≥ L	Bl	2
Claudin-10	PT, TALH	TJ, Bl	Few S	Bl	+/−
Claudin-14	CD	TJ	L?	Ap?	1

Antibodies to claudins 8, 11, 16 and 19 failed to stain mouse kidney paraffin sections satisfactorily.

Tubule segment localization in normal kidney: summary of data from control (wt/wt) mice staining in this study and from published reports [13; 14; 16; 17]. For examples of claudin-7 localization in wild-type mice, please refer to Supplemental Fig. S2 BC, Bowman's capsule; PT, proximal tubule; uTDL, upper thin descending limb; lTDL, lower thin descending limb; ThAL, thin ascending limb; TALH, thick ascending limb; DCT, distal convoluted tubule; CNT, connecting tubule; CD, collecting duct.

TJ, tight junction; Ap, apical plasma membrane; Bl, basolateral plasma membrane

⁴

S, small cyst; I, intermediate cyst; L, large cyst

⁵

Comparison of staining intensity in positive small or intermediate cysts of nl/nl mice, relative to tubules in wild-type mice: 2, similar intensity; 1, slightly weaker intensity; +/−, much weaker intensity; 0, no staining.