Figure 5.

The conditioned medium of P. yoelii-infected erythrocytes modulates DC cytokine production. (A-C) DCs were incubated with medium alone (control), the conditioned medium of uninfected (CM-RBCs) or P. yoelii-infected (CM-iRBCs) erythrocytes for 24 h in the absence (grey bars) or presence (black bars) of 100 ng/ml LPS. (A) PGE₂, (B) IL-6 or (C) IL-12 p70 concentrations were determined in the incubation medium by ELISA. Error bars represent the standard deviation of 3 averaged independent experiments. *, represents significant differences in IL-6 or PGE₂ production by DCs in the presence of CM-iRBCs when compared to production in the presence of CM-RBCs. **, represents significant differences in IL-12 production by DCs in the presence of CM-iRBCs with LPS when compared to production in the presence of CM-RBCs with LPS.