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. Author manuscript; available in PMC: 2009 Nov 1.
Published in final edited form as: Eur J Immunol. 2008 Nov;38(11):3200–3207. doi: 10.1002/eji.200838614

Figure 5.

Figure 5

Phosphorylation kinetics of AKT following TCR stimulation. (A) Thymocytes from PTEN transgenic (Tg) or WT littermates were stimulated with plate-bound anti-CD3/CD28 antibodies for the indicated time points. Cells were fixed and stained with antibodies specific for CD4, CD8, TCRβ and phospho-AKT. (B) Thymocytes from PTEN-deficient mice (PTENflox/flox/lck-Cre) [15] or wild-type littermates were stimulated with plate-bound anti-CD3/CD28 antibodies for the indicated time points. Cells were fixed and stained with antibodies specific for CD4, CD8 and phospho-AKT. Cells were analyzed by flow cytometry. The experiments were done more than three times with similar results.