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. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: Liver Int. 2008 May 19;29(1):63–72. doi: 10.1111/j.1478-3231.2008.01793.x

Figure 6.

Figure 6

Curcumin does not block iron uptake mediated by 8-hydroxyquinoline. Cells were pulsed with calcein-AM, rinsed, and incubated at 37 °C for 2 hours in media containing 10 μM 8-hydroxyquinoline and: no iron (8HQ only), 200 μM FAC (FAC/8HQ), or 200 μM FAC and 50 μM curcumin (FAC/8HQ + curcumin). Control fields without calcein were used to define background for each condition. The calcein fluorescence from 6 distinct fields per condition was quantified as described under Materials and Methods, and mean values +/− s.e. are presented. This experiment was repeated twice with similar results.