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. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: Liver Int. 2008 May 19;29(1):63–72. doi: 10.1111/j.1478-3231.2008.01793.x

Figure 7.

Figure 7

Desferoxamine, but not curcumin, blocks iron uptake mediated by 8-hydroxyquinoline. Cells in 96 well plates were pulsed with calcein-AM, and then incubated at 37 °C for 2 hours in media containing either no iron (light bars), 50 μM FAC (medium bars), or 200 μM FAC (dark bars). Test wells contained 10 μM 8-hydroxyquinoline alone (8HQ), or 10 μM 8-hydroxyquinoline and either: 10 μM curcumin (+ 10 curc), 50 μM curcumin (+ 50 curc), 50 μM desferoxamine (+ 50 dfo), or 50 μM vitamin E (+ 50 VitE). Calcein fluorescence was quantified as described under Materials and Methods. Values (normalized to cells treated with calcein and 8-hydroxyquinoline only) are reported as means +/− se, using data from 3 independent experiments.