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. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: Liver Int. 2008 May 19;29(1):63–72. doi: 10.1111/j.1478-3231.2008.01793.x

Figure 9.

Figure 9

Curcumin blocks multiple iron-sensitive cell signaling pathways. Confluent cells were left untreated (none), or treated with 10 μM 8-hydroxyquinoline (shaded bar) and either 50 μM ammonium citrate (50 AC), 200 μM ammonium citrate (200 AC), 50 μM FAC (50 FAC), 50μM FAC and 50 μM curcumin (50 + curc), 200 μM FAC (200 FAC), or 200 μM FAC and 50 μM curcumin (200 + curc) for the indicated number of hours. Total cell lysates were analyzed by western blotting with antibodies to: jnk dually phosphorylated at thr183/tyr185 (phospho-jnk), c-jun phosphorylated at ser63 (phospho-cjun), p38 dually phosphorylated at thr180/tyr182 (phospho-p38), the p65 subunit of NF-kappaB phosphorylated at ser536 (phospho-p65), or glyceraldehyde 3-phosphate dehydrogenase (GAPDH) as loading control. Blots using antibodies that recognize each protein independently of phosphoylation state (total) are also shown. All panels are from a single gel. The results are representative of at least three similar experiments.