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. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: Mol Reprod Dev. 2009 Feb;76(2):173–182. doi: 10.1002/mrd.20943

Figure 4. Differential utilization of Nanog cis-regulatory elements in F9 and P19 EC cells.

Figure 4

(A) Effect of mutation of the HMG/POU (HP) cassette and PRE-2 on Nanog promoter activity in both F9 and P19 EC cells. (B) Effect of PRE-1 mutation or −812 to −221 truncation of the 5′ flanking region of the Nanog on Nanog promoter in both F9 and P19 EC cells. (C) Effect of − 812 to −221 truncation on Nanog promoter activity in differentiated F9 and P19 cells. In (A), (B), and (C) the cells were transiently transfected and assayed as described in Materials and Methods. Reporter activity was normalized to that of ß-galactosidase and the activity of Nanog −812/+171 was set to 1. Mutant or truncated promoter/reporter construct activity is shown relative to Nanog −812/+171. The data shown represents the mean and standard deviation of duplicate samples from a representative experiment. Each experiment was repeated twice and similar results were obtained.