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. Author manuscript; available in PMC: 2009 Jul 1.
Published in final edited form as: Nat Cell Biol. 2008 Dec 7;11(1):27–35. doi: 10.1038/ncb1809

Figure 4. Temporal regulation of kinetochore-microtubule dynamics.

Figure 4

a, Differential interference contrast (D.I.C.) and time-lapse fluorescent images of prometaphase spindles in untreated (Control) and Kif2b-deficient (Kif2b RNAi) U2OS cells before (Pre-PA) and at the indicated times (sec) after activation (Post-PA) of GFP-tubulin fluorescence. Scale bar, 5 μm. b, Normalized fluorescence intensity over time after photoactivation of spindles in untreated (filled squares), MCAK-deficient (filled circles), and Kif2b-deficient (white squares) prometaphase and metaphase cells. Datapoints represent mean ± s.e.m, n = 5 to 11 cells. c, Calculated kMT half-life under different conditions. Error bars represent S.E. from the regression analysis in b.