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. Author manuscript; available in PMC: 2009 Jul 1.
Published in final edited form as: Nat Cell Biol. 2008 Dec 7;11(1):27–35. doi: 10.1038/ncb1809

Figure 5. Suppression of chromosome mis-segregation in cancer cell lines.

Figure 5

a, b, Percent of anaphase cells with lagging kinetochores and average numbers of lagging chromosomes in anaphase of U2OS cells from representative experiments. Clone 5 and clone 8 are from Table 1. Bars represent mean ± s.e.m, n = 150 cells, 3 experiments (a) and bars represent values from 100 cells, 2 experiements (b), *, p < 0.05, t-test. c, Examples of a proper chromosome segregation in U2OS cells expressing GFP-MCAK (left panel) and mis-segregation event in U2OS cells expressing GFP-Kif2a (right panel) using FISH with probes for chromosomes 2 (green) and 3 (red) and DNA stained with DAPI (blue). Scale bars, 10 μm. d, Mis-segregation rates per chromosome per mitosis in untreated U2OS cells and U2OS cells overexpressing GFP-Kif2a, GFP-Kif2b, GFP-MCAK, or GFP-tubulin as indicated. Clone 5 and clone 8 are from Table 1. n > 660 cells. *, p < 0.05, Chi-square test. e, Model for temporal regulation of kMT dynamics. At low inter-kinetochore tension during prometaphase, the Aurora B kinase activity gradient recruits Kif2b to kinetochores and inhibits centromeric MCAK. The tension generated upon biorientation causes sister kinetochores to exceed the boundaries of the Aurora B kinase activity gradient releasing Kif2b from kinetochores and activating a subset of MCAK in the outer centromere. Kinetochores are shown in grey and microtubules are shown in green.