Fig. 1.

Experimental design. Adult male mice underwent adeno-associated viral-mediated (AAV)VEGF or AAVlacZ gene transfer in the parenchyma of the ipsilateral hemisphere of the brain for 2 wk. A minipump was then implanted intraventricularly in the left hemisphere of the brain. Hydralazine, nicardipine, or saline was consistently infused through the minipump for an additional 2 wk. Tissue samples were collected 4 wk after euthanizing animals. The red dot indicates the location of AAV vector injection; the green dot indicates the location of micropump implantation.