Fig. 4.

Cardiogenic cells loaded with quantum dots (QDs) and implanted on an ECM patch in the canine RV colocalize with sarcomeric α-actinin and exhibit sarcomere structures. A: low-power hematoxylin and eosin image of the patch implant region. Myocardium exists at the edges of the sample and intermixes with fibrous tissue toward the edge of the patch. B: QD 655-labeled cardiogenic cells were found in sarcomeric α-actinin-positive (QD 705) cells in the interior of the patch region. B, inset: QD 655-labeled cardiogenic cells with striations detected by sarcomeric α-actinin staining. The approximate sarcomere length in a cardiogenic cell is labeled. C: QD 655-labeled cardiogenic cells were also found in the border region and appeared to have an alignment similar to neighboring myocytes. D: an example of positive sarcomeric α-actinin staining (QD 705) in normal myocardium.