Fig. 3.

ANG II downregulates ACE2 mRNA in neonatal cardiac myocytes and fibroblasts. Myocytes (A) and cardiac fibroblasts (B) in serum-free media were incubated for 12 h with either 100 nM ANG II, in the presence or absence of 1 μM losartan (Los), with 1 μM losartan alone, or with 10 nM endothelin (ET)-1. ACE2 mRNA was measured by RT real-time PCR; n = 5. *P < 0.01 compared with Control (Con), in the absence of ANG II, losartan, or ET-1.