Fig. 6.

Atrial natriuretic peptide (ANP) blocks the ANG II-mediated reduction in ACE2 mRNA. Myocytes in serum-free media containing 0.5 mM EDTA were incubated with 100 nM ANG II, in the presence or absence of 100 nM ANP. ACE2 mRNA was measured by RT real-time PCR; n = 5–6. *P < 0.01 compared with the Control, in the absence of ANG II or ANP.