Fig. 3.

H₂O₂ affects phosphorylation and activation of nitric oxide (NO) synthase (NOS)3 in HCMVEC. HCMVEC were treated with 500 μM H₂O₂ for the indicated time period with or without intermittent medium replacement 10 min after stimulation, and the status of NOS3 Ser-1177 (A) and Thr-495 (B) phosphorylation was determined by Western blotting. C: experimental data from A and B are presented as the ratio between NOS3 p-Ser-1177 and p-Thr-495. Blots represent 1 out of 3 separate experiments. Results are means ± SE from 3 independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001 vs. untreated control cells. eNOS, Endothelial NOS.