Fig. 6.

A typical nitrotyrosine (N-Tyr) blot shows bands migrating at 30 and 50 kDa (A, see arrows), and N-Tyr expression is significantly higher in I/R compared with the CTL and anti-TNF-α groups (B and C). Values are means ± SE; n = 3. *P < 0.05 vs. sham mice. #P < 0.05 vs. I/R mice. D–F: immunohistochemistry for N-Tyr, a marker of peroxynitrite formation, of representative sections of coronary arterioles from sham mice (D), I/R mice (E), and I/R + anti-TNF-α (before reperfusion) (F) shows chemiluminescence observed. All images were taken at ×40 magnification.