Fig. 4.

Western immunoblot analyses for histone H₃ and cytochrome c as well as caspase-3 activity. A: representative Western immunoblots showing the expression of total and acetyl-histone H₃ (Lys⁹ and Lys¹⁴) in the NF. B: densitometric analysis of acetyl-histone H3 (Lys⁹ and Lys¹⁴). C: representative Western immunoblots showing the expression of cytochrome c and GAPDH in each fraction. D: densitometric analysis of cytochrome c/GAPDH in the CF. E: caspase-3 activity in the CF. MF, mitochondrial fraction. Data are expressed as means ± SE. *P < 0.05 vs. the corresponding AL group.