Fig. 2.

In situ concentrations of myosin light chain (MLC₂₀) and calmodulin in ovine carotid arteries. Artery homogenates were loaded on 15% SDS-PAGE gels along with varying masses of calibrated standards for either MLC₂₀ (left) or calmodulin (right) in adjacent lanes. The integrated density values of each protein were converted to their respective masses by using their respective standard curves and then normalized to intracellular volume using previously published ratios of intracellular water to total cellular protein. Values indicate in situ concentrations of calmodulin and MLC₂₀ for both fetal and adult arteries. Error bars indicate SE for the number of animals indicated in parentheses. There was a significant difference between the fetal and adult values at the P < 0.05 level for calmodulin.