Fig. 5.
Collapse of mitochondrial membrane potential (ΔΨm) after doxorubicin treatment in H9c2 cells. Cells plated on coverslides were maintained in medium with or without 1 μM doxorubicin for 24 h. The cells were stained with 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolocar bocyanine iodide (JC-1) and examined under fluorescence microscopy for red JC-1 aggregates or green JC-1 monomers, as described in materials and methods. Typical images are shown. Magnification scale, 20 μm. Ratio of normal ΔΨm and collapsed ΔΨm is presented at bottom. Data in histograms represent means ± SE from 10 experiments in each group. *P < 0.001, compared with control.