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Neither doxorubicin nor U-0126 produced significant changes on JNK and p38 MAPK proteins in H9c2 cells. Cells were pretreated with or without 20 μM U-0126 for 1 h and then in the presence or absence of 1 μM doxorubicin for 24 h. Whole cell lysates were processed for Western blot analysis of p-JNK, JNK, p-p38, and p38 MAPK. Representative Western blots are shown. Data in histograms represent means ± SE from 6 experiments.
