Table 1.

Effects of U-0126 and PFT-α on cell apoptosis and activation of caspases and PARP

	Control	Doxorubicin
None		U-0126	PFT-α
Apoptosis, %	4.64±0.30	36.10±1.75*	9.67±0.41*†	10.58±0.64*†
Active caspase-3	1.00±0.11	8.40±0.15*	2.54±0.14*†	2.78±0.13*†
Cleaved caspase-9	1.00±0.10	3.74±0.07*	1.71±0.09*†	1.58±0.09*†
Cleaved PARP	1.00±0.09	4.93±0.11*	2.13±0.10*†	2.14±0.11*†

Values are means ± SE; n = 6 experiments. Apoptosis denotes the percentage of terminal deoxynucleotidyl transferase dUTP-mediated nick-end labeling (TUNEL)-positive apoptotic cells in each experimental condition. Values of active caspase-3, cleaved caspase-9, and cleaved poly(ADP-ribose) polymerase (PARP) were derived from optical density readings of the Western blots normalized to a control sample for each of the proteins.

^*P < 0.05, compared to control;

^†P < 0.05, compared to doxorubicin alone. PFT, pifithrin.