Table 2.

Effects of U-0126 and PFT-α on phospho-ERK1/2, phospho-p53, and Bcl-2 family proteins

	Control	Doxorubicin
None		U-0126	PFT-α
Phospho-ERK1/2	1.00±0.05	7.9±0.32*	2.50±0.22*†	6.86±0.22*
Phospho-p53	1.00±0.07	4.56±0.11*	2.39±0.10*†	2.10±0.07*†
Bax	1.00±0.07	8.62±0.28*	4.87±0.19*†	4.59±0.24*†
Bcl-2	1.00±0.02	0.51±0.02*	0.86±0.02*†	0.90±0.03*†
PUMA-α	1.00±0.13	9.56±0.10*	2.74±0.35*†	2.78±0.22*†

Values are means ± SE; n = 6 experiments. Optical density for the Western blots was normalized to a control sample for each of the proteins.

^*P < 0.05, compared to control;

^†P < 0.05, compared to doxorubicin alone. PUMA, p53 upregulated modulator of apoptosis.