Fig. 2.
Detection of FcαRI proteins in human platelets, which were purified as described in Materials and Methods. The purified platelets (109) from three normal, healthy donors (Lanes 1–3) were lysed, and cell lysates were separated in 12% SDS-PAGE under reducing conditions. FcαRI proteins were detected on four separate blots with three anti-FcαRI mAb (A77, A59, and MIP8a) and rabbit pAb against the FcαRI cytoplasmic domain in Western blot analysis. FcαRI proteins were shown as multiple protein bands with a molecular weight (Mr) between 50 and 70 kDa. IB, Immunoblot.