Figure 4.

Impairment of PTEN degradation in LMP-2^−/− hearts after IPC. Isolated hearts from LMP-2^+/+ and LMP-2^−/− mice were subjected to control (nonischemia) or IPC (I-10/R-5). A) Representative Western blots of PTEN, p-Akt, and Akt. B) IPC significantly decreased PTEN protein levels in LMP-2^+/+ hearts, but not in LMP-2^−/− hearts. C) Phosphorylated downstream Akt was significantly increased in LMP-2^+/+ hearts, but not in LMP-2^−/− hearts after IPC. *P < 0.01 vs. CON_WT; n = 3/group.