Figure 7.
Regulation of the immunoproteasome by IPC. Lysates were generated from isolated LMP-2+/+ and LMP-2−/− hearts with or without IPC. A, B): LMP-2 was increased in LMP-2+/+ hearts after IPC but was undetectable in LMP-2−/− hearts. *P < 0.001 vs. CONWT. C, D) MECL-1 was decreased in LMP-2+/+ hearts after IPC. Under basal conditions, MECL-1 was less in LMP-2−/− hearts. *P < 0.01, #P < 0.001 vs. CONWT. E, F) IPC decreased LMP-7 protein levels in LMP-2+/+ heart, and there was less LMP-7 in LMP-2−/− hearts. *P < 0.01, #P < 0.01 vs. CONWT. n = 4/group.