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. 2008 Dec;22(12):4126–4135. doi: 10.1096/fj.08-112326

Figure 3.

Figure 3.

A) Effects of miR-377 on mesangial cell expression of Bcl-xL, PAK1, Smad7 and PPM1A proteins. Indicated are elapsed times after miR-377 transfection. Blots are representative of experiments performed 2–3 times. B) Effects of PPM1A gene knockdown on TGF-β-induced fibronectin production in normal mesangial cells. Blots are representative of experiments performed 4 times. C) MicroRNA-377 reduces expression of luciferase in a reporter containing the 3′-UTR of PAK1. Data are expressed as means ± se of experiments performed in triplicate 24 h following cotransfection. D) MicroRNA-377 increases expression of fibronectin and decreases PAK1 in normal mesangial cells. Blots are representative of experiments performed 4 times. The protein mobilities in the gel are the same as in E. E) Effects of glucose and FCS concentrations and PAK1 gene knockdown on fibronectin expression in normal mesangial cells. Blots are representative of experiments performed twice. F) Expression of PAK1 in glomeruli isolated from 10-wk diabetic and control mice, and normal mesangial cells cultured in 5 and 22.5 mM glucose for 6-wk duration.