TABLE 1.
Oligonucleotides employed in these studies
| Oligonucleotide | Sequence |
|---|---|
| Primers for amplification of miR-377 targets on 3′-UTRsa | |
| P-miR-377 (left) | ACAAAAGTTGCCTTTGTGTGAT |
| P-control (left) | ACAAATGTAGCGTTAGTCTGTT |
| P-oligo-dT (right) | TTTTTTTTTTTTTTTTTTTT |
| qPCR primers | |
| P-actin (left) | AGCCATGTACGTAGCCATCC |
| P-actin (right) | CTCTCAGCTGTGGTGGTGAA |
| P-18S rRNA (left) | ATGGCCGTTCTTAGTTGGTG |
| P-18S rRNA (right) | CGCTGAGCCAGTCAGTGTAG |
| Primers for ChIP assay | |
| ChIPmir-377–1 | ACACTGTATCCTTGGCAGTGG |
| ChIPmir-377–2 | GCGGGATTTGGTACTGAAAA |
| ChIPmir-377–3 | AGGGAAAGGTCAAGGTCAGAA |
| ChIPmir-377–4 | GGGCAGATGGCTACATATTCT |
| Probes for in situ hybridizationa | |
| Probe-miR-377 | ACAAAAGTTGCCTTTGTGTGAT |
| Probe-control | ACAAATGTAGCGTTAGTCTGTT |
| siRNA | |
| SOD1 | UGAUUGGGAUUGCGCAGUAUU |
| GGACAAAUUACAGGAUUAAUU | |
| GCAGGGAACCAUCCACUUCUU | |
| AAGAGAGGCAUGUUGGAGAUU | |
| PAK1 | AAGAAGACCUCCAAUAGUAUU |
| GUAUAUACGAUCUGUGAUU | |
| GAUUGGAGCCGGCAGCAAAUU | |
| CGAAGAAAGAGCUGAUUAUUU | |
| PPM1A | ACAAUAGACUGAACCCUUAUU |
| UCACCAAUAACCAGGAUUUUU | |
| ACACGGCUGUGAUCGGUUUUU | |
| GCAAGCGGAAUGUAAUUGAUU | |
| PcDNA constructs | |
| PC-miR-377 (forward) | TGCTGAGCAGAGGTTGCCCTTGGTGAATTCGCTTTATTTATGTTGAATCACACAAAGGCAACTTTTGTT |
| PC-miR-377 (reverse) | CCTGAACAAAAGTTGCCTTTGTGTGATTCAACATAAATAAAGCGAATTCACCAAGGGCAACCTCTGCTC |
| PC-miR-337 (forward) | TCGAGAAGTTGGGGGGTGGGAACGGCGTCATGCAGGAGTTGATTGCACAGCCATTCAGCTCCTATATGATGCCTTTCTTCACCCCCTTCAA |
| PC-miR-337 (reverse) | GATCTTGAAGGGGGTGAAGAAAGGCATCATATAGGAGCTGAATGGCTGTGCAATCAACTCCTGCATGACGCCGTTCCCACCCCCCAACTTC |
a
Underscores indicate “locked” nucleotides.