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The p50/p105 NFKB1 I/D promoter regulates NFKB1 gene expression. Cytoplasmic proteins were extracted from genotyped homozygous II and DD HUVECs, and immunoblots were performed with monoclonal antibody against p50/p105 proteins. Higher basal p50/p105 protein levels were observed in II homozygote cells compared with DD homozygote cells. Actin was used as an internal control for equal sample loadings. P, passage.
