Fig. 4.

Examples of c-Fos immunoreactivity (Fos-IR) following either the restraint or non-restraint condition for WT and CRF2 KO mice. Restraint or non-restraint were performed 105 min (±5 min) before brain fixation. Unrestrained CRF2 KO mice exhibit significantly lower levels of Fos-IR relative to unrestrained WT mice in cPAG. In addition, with restraint, CRF2 KO mice show significant increases in Fos-IR relative to their unrestrained state in cPAG. These differences in cPAG were photographed on the right side of the brain. PVN shows significant increases in Fos-IR with restraint for both WT and CRF2 KO mice (see Results for more details).