Table 5.
Changes in c-Fos activity in WT and KO lactating dams
| Region | WT | CRF2 KO | Two Way Genotype p-value | ANOVA Restraint p-value | ||
|---|---|---|---|---|---|---|
| Unrestrained (n) | Restrained (n) | Unrestrained (n) | Restrained (n) | |||
| AOM | 11.3±4.8 (3) a | 14.1±7.3 (7) | 0.8±0.4 (5) a,b | 11.0±4.8 (9) # b | 0.245 | 0.267 |
| AOP | 10.0±4.7 (3) a | 14.7±5.3 (7) | 1.20±0.4 (5) a,b | 9.3±3.4 (9) # b | 0.133 | 0.172 |
| VTT | 17.5±0.5 (2) a | 31.8±10.0 (5) | 3.8±0.6 (4) a,b | 21.9±5.6 (8) # b | 0.240 | 0.099 |
| F Cg Cx | 52.3±13.5 (4) | 91.1±28.1 (7) | 27.4±10.9 (7) | 59.9±13.6 (9) | 0.133 | 0.062 |
| IL | 80.3±25.8 (4) | 108.4±26.2 (7) | 41.7±12.8 (7) | 93.2±12.9 (9) | 0.152 | 0.041 |
| NuAcbC | 28.8±4.3 (4) | 65.9±22.8 (7) # | 17.1±4.6 (7) | 52.0±13.3 (9) # | 0.308 | 0.020 |
| NuASh | 26.8±15.9 (4) a | 33.3±15.7 (7) # | 4.9±1.6 (7) a | 19.6±6.2 (9) # | 0.068 | 0.212 |
| MFC | 56.8±21.4 (5) a | 102.3±35.1 (7) | 14.7±5.6 (7) a,b | 89.4±36.7 (9) # b | 0.480 | 0.089 |
| Pir Cx | 113.20±24.5 (5) | 136.7±20.5 (7) | 98.3±34.2 (8) | 117.6±19.9 (9) | 0.525 | 0.423 |
| LS1 | 43.8±14.2 (4) | 45.4±15.9 (7) # | 55.7±17.5 (7) | 55.4±19.7 (9) | 0.446 | 0.756 |
| MS/VDB | 15.6±9.1 (5) | 19.9±8.3 (8) | 19.2±6.1 (6) | 22.8±7.6 (9) # | 0.828 | 0.767 |
| LS2 | 77.6±17.5 (5) | 71.9±15.2 (7) | 52.8±11.2 (8) | 93.6±28.4 (9) # | 0.727 | 0.290 |
| LSV1 | 122.8±15.8 (5) a | 131.9±38.6 (7) | 63.0±12.1 (8) a,b | 136.8±22.0 (9) b | 0.215 | 0.078 |
| MPOM | 28.2±3.0 (5) | 79.9±21.0 (8) | 66.1±21.2 (7) | 62.3±13.3 (8) | 0.582 | 0.201 |
| MPA | 24.0±5.0 (5) | 51.3±10.3 (8) | 42.4±9.0 (7) | 40.5±10.2 (8) | 0.699 | 0.210 |
| LPO | 9.8±3.6 (6) | 36.8±9.9 (8) | 17.4±4.7 (7) | 26.0±6.0 (8) | 0.833 | 0.023 |
| BNSTd | 29.0±6.7(6) | 56.4±9.6 (7) | 21.4±7.0 (8) | 24.6±4.9 (9) | 0.010 | 0.041 |
| BNSTv | 27.5±8.5 (6) | 28.1±4.1 (7) | 13.5±3.6 (8) | 23.2±4.4 (9) | 0.076 | 0.321 |
| Cg Cx | 57.5±26.7 (6) | 127.8±36.8 (6) | 86.3±28.8 (8) | 115.7±35.5 (9) | 0.834 | 0.125 |
| LS3 | 52.3±15.4 (4) | 57.7±13.1 (7) | 28.8±5.5 (8) | 58.3±19.5 (9) # | 0.444 | 0.253 |
| LSV2 | 90.0±34.1 (4) | 118.3±17.4 (7) | 80.5±19.4 (8) | 93.9±15.6 (9) | 0.422 | 0.322 |
| SCN | 63.0±17.4 (6) c | 136.3±23.1 (7) c | 98.7±36.1 (7) | 132.9±15.5 (9) | 0.351 | 0.070 |
| PVN | 48.0±10.7 (5) c | 205.3±39.6 (7) c # | 43.4±9.4 (8) b | 204.4±28.2 (9) # b | 0.962 | <0.001 |
| AHA | 18.0±3.7 (5) | 31.1±5.2 (7) | 10.2±1.7 (6) b | 25.3±3.7 (8) b | 0.100 | 0.002 |
| PVA | 232.4±57.2 (5) | 262.1±51.2 (7) | 161.5±33.5 (8) b | 264.9±14.2 (9) b | 0.528 | 0.419 |
| SON | 16.3±4.9 (6) | 17.3±4.3 (6) | 27.1±14.6 (7) | 17.8±6.2 (9) # | 0.479 | 0.588 |
| CeAmy | 104.8±37.1 (4) | 152.3±32.5 (8) | 61.4±26.9 (8) | 124.7±19.8 (7) | 0.256 | 0.082 |
| MeAmy | 23.8±4.6 (4) | 26.0±6.6 (8) | 15.8±3.6 (8) | 22.8±3.7 (8) | 0.290 | 0.382 |
| LH | 56.3±12.8 (4) | 70.6±9.9 (8) | 46.9±11.1 (8) | 67.9±8.6 (7) | 0.587 | 0.122 |
| VMH | 13.0±6.0 (4) | 25.5±9.8 (6) | 5.8±1.5 (8) b | 30.1±8.6 (7) # b | 0.860 | 0.020 |
| AHP | 85.3±23.1 (4) | 106.6±11.7 (8) | 60.1±9.3 (8) b | 116.3±18.9 (7) b | 0.620 | 0.019 |
| CA1 | 5.3±2.6 (4) | 15.3±9.8 (7) # | 6.6±3.6 (7) | 4.8±1.6 (9) # | 0.439 | 0.487 |
| CA2 | 11.3±1.7 (4) | 16.9±6.3 (7) | 10.4±5.5 (7) | 13.0±3.4 (9) # | 0.657 | 0.440 |
| CA3 | 24.8±0.6 (4) | 31.9±8.7 (7) # | 18.7±4.1 (6) | 22.8±3.9 (9) | 0.222 | 0.363 |
| DG | 44.3±8.7 (4) a | 32.4±8.7 (7) | 18.4±3.8 (7) a | 22.0±2.2 (9) | 0.006 | 0.495 |
| DM | 47.3±19.6 (6) | 117.7±25.9 (7) # | 44.8±8.2 (8) b | 101.2±11.6 (9) b | 0.548 | <0.001 |
| PeF | 26.3±5.6 (6) c | 46.1±6.2 (7) c | 15.3±2.8 (7) b | 50.2±6.2 (9) b | 0.568 | <0.001 |
| PH | 150.0±37.0 (5) | 157.0±17.6 (6) | 115.6±32.6 (7) b | 193.6±19.0 (9) b | 0.886 | 0.139 |
| VTA | 41.4±9.6 (5) a | 39.0±9.5 (7) | 18.5±5.5 (8) a,b | 41.8±8.3 (9) b | 0.344 | 0.322 |
| cPAG | 109.0±5.6 (4) a | 86.7±10.9 (8) | 63.8±8.9 (8) a,b | 110.9±15.8 (9) # b | 0.444 | 0.367 |
| DRD | 89.0±9.9 (4) | 106.9±17.6 (7) | 60.5±11.7 (8) b | 118.7±20.4 (9) b | 0.654 | 0.050 |
| LDTg | 38.6±12.0 (5) | 31.3±7.5 (7) | 24.3±6.1 (7) | 17.1±5.3 (7) | 0.066 | 0.301 |
| PB | 26.0±11.3 (5) | 27.0±9.2 (7) | 19.9±8.5 (7) | 15.2±4.8 (6) | 0.644 | 0.694 |
| LC | 71.8±12.9 (5) | 35.1±14.1 (7) | 30.9±15.7 (7) | 44.1±15.5 (7) | 0.533 | 0.727 |
Mean (±SEM) number of c-Fos-positive cells in brain regions for WT and KO mice that were either unrestrained as a control or exposed to a mild stressor for 15 minute using a two-way ANOVA. Planned comparisons with a one-way ANOVA are also represented.
a
An effect of genotype (p<0.05) between unrestrained WT and KO mice.
b
An effect of restraint stress treatment within KO mice.
c
An effect of restraint stress treatment within WT mice.
Restraint did not alter c-Fos activity in WT mice. Regions with p<0.05 are shown in bold.
cPAG was the only brain region to show a significant effect of genotype x restraint (p=0.017, see Results).
#
data non-normal, analyzed with the Kruskal-Wallis One Way ANOVA on Ranks.