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. Author manuscript; available in PMC: 2009 Nov 1.
Published in final edited form as: Steroids. 2008 Jun 22;73(12):1252–1261. doi: 10.1016/j.steroids.2008.06.004

Figure 1.

Figure 1

Comparison of the relative binding affinity (IC50) of compound 7 for human ERα and ERβ. The binding affinity was also compared with those of E2, RAL and ICI-182,780 (summarized in the accompanying Table below). The relative binding affinity of each chemical was determined by measuring its inhibition of the binding of 10 nM [3H]E2 to the recombinant human ERα and ERβ. Eight concentrations (0.06, 0.24, 0.98, 3.9, 15.6, 62.5, 250, and 1000 nM) of each competing estrogen were tested. The IC50 values for the competing ligands were calculated according to the sigmoid inhibition curves.