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. Author manuscript; available in PMC: 2009 Jan 7.
Published in final edited form as: Mol Microbiol. 2008 Dec;70(6):1487–1501. doi: 10.1111/j.1365-2958.2008.06495.x

Fig. 5.

Fig. 5

Immunoblot analysis of small proteins predicted on the basis of potential RBSs with high information content.

A. Small proteins predicted in an initial search for genes with canonical RBSs.

B. Small proteins predicted using an information theory-based RBS search for open reading frames. For both (A) and (B), whole cell extracts of MG1655 cells grown to (E) exponential and (S) stationary phase in LB medium were analyzed as in Fig. 1. Again the star (*) indicates the band corresponding the fusion protein. The caveats of the marker lane and exposure times are as for Fig. 1. Given the need for longer exposure times, some background bands were detected for the immunoblots in second row of (B).