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LeuRS CP1 deletion design. (A) The aminoacylation core and CP1 domain of the E. coli LeuRS (ecLeuRS) homology model (31) are orange and blue, respectively. (B) Sequence of ecLeuRS and yeast mitochondrial LeuRS (ymLeuRS) with marked site of CP1 deletion (blue) that was replaced by insertion of three- and two-alanine peptides (black), respectively.
