Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2008 Nov 19;105(49):19223–19228. doi: 10.1073/pnas.0809336105

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2008 by The National Academy of Sciences of the USA

PMC Copyright notice

Fig. 2. — Aminoacylation and deacylation activity. Leucylations by E. coli LeuRS and ecΔCP1 (A) or ymLeuRS and ymΔCP1 (B) were carried out by using 4 μM tRNA^Leu and 50 nM enzyme. Deacylation reactions for E. coli LeuRS (C) and ymLeuRS (D) wild type and ΔCP1 deletion mutants contained ≈6.5 μM [³H]Ile-tRNA^Leu and 100 nM enzyme. Isoleucine mischarging by E. coli LeuRS (E) and ymLeuRS (F) wild type and ΔCP1 deletion mutants incorporated 21 μM [³H]isoleucine (166 Ci/mmol) and 1 μM enzyme. Editing-defective E. coli T252Y LeuRS (34) and TT/VV ymLeuRS (5) were used as positive controls. ■, E. coli LeuRS wild type (ecWT); (▲, E. coli LeuRS ΔCP1 (ecΔCP1); □, ymLeuRS wild type (ymWT); ◇, ymLeuRS ΔCP1 (ymΔCP1); , E. coli T252Y LeuRS (ecT252Y); ♦, ymLeuRS TT/VV; ●, no enzyme control (no E). Error bars represent triplicated reactions.

Inline graphic — Aminoacylation and deacylation activity. Leucylations by E. coli LeuRS and ecΔCP1 (A) or ymLeuRS and ymΔCP1 (B) were carried out by using 4 μM tRNA^Leu and 50 nM enzyme. Deacylation reactions for E. coli LeuRS (C) and ymLeuRS (D) wild type and ΔCP1 deletion mutants contained ≈6.5 μM [³H]Ile-tRNA^Leu and 100 nM enzyme. Isoleucine mischarging by E. coli LeuRS (E) and ymLeuRS (F) wild type and ΔCP1 deletion mutants incorporated 21 μM [³H]isoleucine (166 Ci/mmol) and 1 μM enzyme. Editing-defective E. coli T252Y LeuRS (34) and TT/VV ymLeuRS (5) were used as positive controls. ■, E. coli LeuRS wild type (ecWT); (▲, E. coli LeuRS ΔCP1 (ecΔCP1); □, ymLeuRS wild type (ymWT); ◇, ymLeuRS ΔCP1 (ymΔCP1); , E. coli T252Y LeuRS (ecT252Y); ♦, ymLeuRS TT/VV; ●, no enzyme control (no E). Error bars represent triplicated reactions.