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. 2008 Nov 19;105(49):19223–19228. doi: 10.1073/pnas.0809336105

Fig. 5.

Fig. 5.

Amino acid and tRNA-dependent ATPase activity of E. coli wild-type LeuRS and CP1 deletion mutant. Reaction mixtures contained 10 μM tRNALeu or a tRNA analog with 2′-deoxyadenosine substituted for the A76 base (2′dA) and 1 μM enzyme. Shown is ATP consumption during ATPase reaction by E. coli wild type (A) and CP1 deletion mutant (B). □, plus isoleucine and tRNA; △, plus isoleucine and 2′dA tRNA; ◇, plus 2′dA tRNA only; ○, plus isoleucine only. Error bars represent triplicated reactions for all time points.