Fig. 3.

Decreased expression of adipogenic genes in MLL3^Δ/Δ mice and partially impaired adipogenic potential of E13.5 MLL3^Δ/Δ MEFs. (A–C) Expression of ApoC1 and RBP4 (A) and MLL3/4 (B) and aP2, adiponectin, C/EBPα and PPARγ (C) in E13.5 WT and MLL3^Δ/Δ WAT or BAT was measured by Q-PCR (n = ≈3–5). We also measured expression of aP2 as positive controls for WAT and BAT (B). (D) E13.5 WT and MLL3^Δ/Δ MEFs were induced to differentiate to adipocytes. Experiments were repeated 3 times, and MLL3^Δ/Δ MEFs reproducibly showed ≈80% efficiency of WT MEFs in adipogenesis. (Magnification: ×4.)