Fig. 2.

Representative chevron plots. (A) Three chevrons of pseudo-wild-type PSD-95 PDZ3 (F337W). The solid line for the pseudo-wild-type in A is the average fit to the three independent experiments. (B–D) Examples of high (B), low (C), and average (D) Φ values. Only one chevron plot was measured for each mutant. The solid lines are obtained from a global fit of all kinetic data (all mutants and wild type), with shared m values (m_D-‡1 = 0.84 ± 0.01, m_N-‡1 = 0.66 ± 0.01, and m_p = 0.50 ± 0.01 kcal·mol⁻¹·M⁻¹; errors are from the global fitting).