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. 2008 Nov 25;105(49):19241–19246. doi: 10.1073/pnas.0804774105

Table 1.

Experimental Φ values for PSD-95 PDZ3

Protein region Mutant ΔΔGD-N, kcal· mol−1 ΦTS1 ΦTS2
Mutation compared with wild type
β1 I314V −0.44 ± 0.08*
β1 I316A 3.5 ± 0.2 0.28 ± 0.04 0.52 ± 0.03
Loop L323A 1.87 ± 0.08 −0.20 ± 0.03 0.38 ± 0.05
β2 F325A 4.9 ± 1.0 0.36 ± 0.13 0.67 ± 0.07
β2 I327V 0.96 ± 0.07 0.25 ± 0.03 0.52 ± 0.09
β2 V328A 0.32 ± 0.08*
β3 I336V 0.45 ± 0.07*
β3 I336A 1.35 ± 0.08 0.01 ± 0.03 0.53 ± 0.06
β3 I338A 2.2 ± 0.1 0.15 ± 0.03 0.53 ± 0.04
β3 F340A 0.12 ± 0.08*
β3 I341V −0.16 ± 0.08*
β3 I341A 1.57 ± 0.08 −0.08 ± 0.03 0.42 ± 0.05
α1 P346G 1.33 ± 0.07 −0.05 ± 0.03 0.27 ± 0.07
α1 A347G 1.32 ± 0.07 −0.05 ± 0.03 0.20 ± 0.07
Loop L353A 2.24 ± 0.09 0.15 ± 0.02 0.50 ± 0.04
β4 I359V 0.94 ± 0.07 0.36 ± 0.04 0.60 ± 0.09
β4 V362A 1.68 ± 0.08 0.60 ± 0.02 0.87 ± 0.06
α2 H372A −1.3 ± 0.1 0.87 ± 0.05 0.77 ± 0.09
α2 E373A 0.05 ± 0.08*
α2 E373G 0.65 ± 0.08 0.50 ± 0.06 0.62 ± 0.14
α2 A375G 0.98 ± 0.07 0.79 ± 0.05 1.04 ± 0.13
α2 A376G 1.35 ± 0.07 0.55 ± 0.03 0.84 ± 0.08
α2 I377A 0.00 ± 0.08*
α2 I377G 1.21 ± 0.07 0.58 ± 0.03 0.75 ± 0.08
α2 A378G 2.4 ± 0.1 0.34 ± 0.02 0.60 ± 0.04
α2 L379A 1.52 ± 0.07 0.59 ± 0.03 0.82 ± 0.07
α2 K380A −0.58 ± 0.08 0.77 ± 0.09 0.69 ± 0.16
α2 K380G 0.53 ± 0.07 0.18 ± 0.07 0.75 ± 0.19
Turn A382G 1.19 ± 0.08 0.38 ± 0.03 0.59 ± 0.07
β6 V386A 2.00 ± 0.07 0.16 ± 0.02 0.56 ± 0.04
β6 I388V 1.44 ± 0.07 0.35 ± 0.02 0.56 ± 0.06
β6 Y392A 1.51 ± 0.08 0.17 ± 0.03 0.96 ± 0.08
Val→Ala or Ala→Gly
β3 V336A 0.90 ± 0.08 0.00 ± 0.05 0.33 ± 0.10
β3 V341A 1.73 ± 0.08 −0.03 ± 0.03 0.45 ± 0.05
α2 A373G 0.60 ± 0.08 0.42 ± 0.06 0.51 ± 0.14
α2 A377G 1.21 ± 0.08 0.66 ± 0.04 0.78 ± 0.08
α2 A380G 1.11 ± 0.08 0.49 ± 0.03 0.72 ± 0.09

The standard errors of curve fitting for the kinetic parameters were similar to standard deviations, as determined from three independent kinetic measurements on the pseudo-wild-type PDZ3 (Fig. 2A). Therefore, fitting errors of kf, ku, and Kp were used to calculate the propagated errors in Φ and Δ Δ GD-N as described (50).

*The ΔΔ GD-N value is too low to calculate an accurate Φ value.